The Nextera XT library preparation kit is optimized for 1 ng double-stranded genomic DNA. Limited cycle PCR to add sequencing primer sequences and indexes How well the sample is tagmented determines the success of the library prep. Tagmentation to fragment and add partial adapters C. Nextera XT transposome with partial adapters is combined with template DNA Call out. The following figure illustrates the process. The first step in library prep is the tagmentation reaction, which involves the transposon cleaving and tagging of the double-stranded DNA with a universal overhang. 1 Nextera XT Library Prep: Tips and Troubleshooting Sample Input: Success Relies on Quality and Quantity Sample input is the most important factor in a successful Nextera XT library prep.
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